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Apart from Phony-constructive final results, heterophile antibodies can cause falsely small success whenever they bind on the variable regions on the seize topanwin login antibody, mimicking the antigen being measured and blocking troponin from binding.[89] The most commonly employed technique for minimizing the influence of HAMA on business immunoassays may be the addition of non-immune mouse immunoglobulin (IgG).
The captured troponin is then reacted by using a 2nd antibody and, in some assays, a 3rd antibody coupled to an indicator molecule.[36] The assays vary from one another by the categories of antibody employed, through the epitopes to which they bind, and by the type of indicator molecule that is definitely utilised.[forty six]
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